Posts

Electrocatalytic Microdevices: A Miniaturized Revolution in Electrochemical Analysis

-
Introduction: Electrocatalytic microdevices are cutting-edge tools that integrate microscale technologies with electrocatalysis, enabling highly sensitive and rapid electrochemical analysis. This protocol outlines the steps for fabricating and utilizing on-chip electrocatalytic microdevices, making it accessible for bloggers and enthusiasts interested in the field of microscale electrochemistry.   Materials: Substrate material (e.g., glass, silicon) Conductive material (e.g., gold, platinum) Insulating material (e.g., photoresist) Electrode material (e.g., carbon nanotubes, metal nanoparticles) Microfabrication tools (e.g., photolithography equipment) Potentiostat Electrolyte solution Sample solution for analysis Protocol:   1. Substrate Preparation: -Clean the substrate thoroughly to remove any contaminants. -Deposit a conductive layer (e.g., gold) on the substrate using deposition techniques like sputtering or evaporation. 2. Photolithography:  - Apply a layer of insulating material
Post a Comment
Read more

Fluorescent Labeling and Tracking of Extracellular Matrix: Illuminating the Molecular Landscape In Vivo

-
Introduction: Understanding the dynamics of the extracellular matrix (ECM) is crucial for unraveling various physiological and pathological processes. In vivo fluorescent labeling and tracking of ECM components provide valuable insights into tissue remodeling, wound healing, and disease progression. This protocol employs fluorescent markers to visualize ECM components in real-time, offering a powerful tool for researchers studying tissue microenvironments. Materials: - Fluorescent dyes/proteins specific to ECM components (e.g., collagen, fibronectin) - Animal model (mouse, zebrafish, etc.) expressing ECM-specific fluorescent proteins (optional) - Anesthesia suitable for the chosen animal model - Microscope equipped with appropriate fluorescence filters - Imaging chamber for live animal imaging - Surgical tools for exposing the tissue of interest - Sterile saline solution for tissue hydration - Sutures or wound clips for tissue closure (if applicable) Protocol: 1. Selection of ECM-Speci
Post a Comment
Read more

RNA isolation using TRIZOL sigma reagent from plant tissue

-
Image
In this pictorial abstract, you will learn how to do RNA isolation from plant tissue using TRIzol sigma. The abstract describes steps followed during RNA isolation with TRIzol. RNA isolate from plant tissue could be further used in performing a quantitative study of genes, preparing cDNAs, and do profiling of RNA. We hope the description is easy to understand and useful for all. RNA isolation using TRIZOL sigma reagent from plant tissue
Post a Comment
Read more

DNA isolation from plant tissue

-
Image
In this pictorial abstract, you will learn how to do DNA extraction from plant tissue manually. The abstract describes steps followed during genomic DNA isolation. Isolation of DNA from plants is useful during the genotyping of transgenic plants. It also gives us ideas of genomic segregation at different generations. We hope all the information is easy to understand and useful for all. DNA isolation from plant tissue
Post a Comment
Read more

Preparing competent cells using calcium chloride method

-
Image
In this pictorial abstract, you will learn how to prepare competent bacterial cells using the calcium chloride method. The abstract describes steps followed during competent cell preparation. The competent cell is used to transform a plasmid, obtained from the desired strain of bacteria using plasmid isolation methods. Genes cloned into that plasmid are also functionally analyzed in the competent bacterial cells. We hope the description is easy to understand and useful for all. Preparing competent cells using a calcium chloride method
Post a Comment
Read more

Plasmid isolation by alkaline lysis method

-
Image
In this pictorial abstract, you will learn the manual method used for plasmid isolation from the bacterial cell. The abstract describes the step followed during the manual method . However, Many plasmid isolation kits are available in the market, manufactured by biotech companies at a very high price. But the use of a  manual method for plasmid isolation is more effective . We hope that the procedure described in this abstract will be useful for all. Plasmid isolation by alkaline lysis method
Post a Comment
Read more