How to prepare

  1. How to prepare EDTA solution                             Coming soon
  2. How to prepare TE buffer                                      Coming soon
  3. How to prepare CTAB buffer
  4. How to prepare Luria Bertani buffer
    Luria Bertani (LB)(1 Litre)

Chemical

Quantity

Peptone

10 gm

Sodium Chloride

10 gm

Yeast extract

5 gm

LB broth:  The chemicals given in the table dissolve in 900 ml of water, set the pH to 7, makeup the volume upto 1litre and autoclaved it

LB Agar: 15g of Bacteriological agar add into 900 ml of water, makeup the volume upto 1litre and then autoclaved it.


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