Protocols

In this pictorial abstract, you will learn how to do RNA isolation from plant tissue using TRIzol sigma. The abstract describes steps followed during RNA isolation with TRIzol. RNA isolate from plant tissue could be further used in performing a quantitative study of genes, preparing cDNAs, and do profiling of RNA. We hope the description is easy to understand and useful for all. RNA isolation using TRIZOL sigma reagent from plant tissue

In this pictorial abstract, you will learn how to do DNA extraction from plant tissue manually. The abstract describes steps followed during genomic DNA isolation. Isolation of DNA from plants is useful during the genotyping of transgenic plants. It also gives us ideas of genomic segregation at different generations. We hope all the information is easy to understand and useful for all. DNA isolation from plant tissue

In this pictorial abstract, you will learn how to prepare competent bacterial cells using the calcium chloride method. The abstract describes steps followed during competent cell preparation. The competent cell is used to transform a plasmid, obtained from the desired strain of bacteria using plasmid isolation methods. Genes cloned into that plasmid are also functionally analyzed in the competent bacterial cells. We hope the description is easy to understand and useful for all. Preparing competent cells using a calcium chloride method

In this pictorial abstract, you will learn the manual method used for plasmid isolation from the bacterial cell. The abstract describes the step followed during the manual method . However, Many plasmid isolation kits are available in the market, manufactured by biotech companies at a very high price. But the use of a  manual method for plasmid isolation is more effective . We hope that the procedure described in this abstract will be useful for all. Plasmid isolation by alkaline lysis method