In this pictorial abstract, you will learn how to prepare competent bacterial cells using the calcium chloride method. The abstract describes steps followed during competent cell preparation. The competent cell is used to transform a plasmid, obtained from the desired strain of bacteria using plasmid isolation methods. Genes cloned into that plasmid are also functionally analyzed in the competent bacterial cells. We hope the description is easy to understand and useful for all.
Preparing competent cells using a calcium chloride method
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