Protocols

Introduction: Electrocatalytic microdevices are cutting-edge tools that integrate microscale technologies with electrocatalysis, enabling highly sensitive and rapid electrochemical analysis. This protocol outlines the steps for fabricating and utilizing on-chip electrocatalytic microdevices, making it accessible for bloggers and enthusiasts interested in the field of microscale electrochemistry.   Materials: Substrate material (e.g., glass, silicon) Conductive material (e.g., gold, platinum) Insulating material (e.g., photoresist) Electrode material (e.g., carbon nanotubes, metal nanoparticles) Microfabrication tools (e.g., photolithography equipment) Potentiostat Electrolyte solution Sample solution for analysis Protocol:   1. Substrate Preparation: -Clean the substrate thoroughly to remove any contaminants. -Deposit a conductive layer (e.g., gold) on the substrate using deposition techniques like sputtering or evaporation. 2. Photolithography:  - Apply a layer of insulating material

Introduction: Understanding the dynamics of the extracellular matrix (ECM) is crucial for unraveling various physiological and pathological processes. In vivo fluorescent labeling and tracking of ECM components provide valuable insights into tissue remodeling, wound healing, and disease progression. This protocol employs fluorescent markers to visualize ECM components in real-time, offering a powerful tool for researchers studying tissue microenvironments. Materials: - Fluorescent dyes/proteins specific to ECM components (e.g., collagen, fibronectin) - Animal model (mouse, zebrafish, etc.) expressing ECM-specific fluorescent proteins (optional) - Anesthesia suitable for the chosen animal model - Microscope equipped with appropriate fluorescence filters - Imaging chamber for live animal imaging - Surgical tools for exposing the tissue of interest - Sterile saline solution for tissue hydration - Sutures or wound clips for tissue closure (if applicable) Protocol: 1. Selection of ECM-Speci